4. "Insilico analysis and 3d structure prediction of mitochondrial RHO GTPase 2 protein of Zebra Fish (Danio rerio)", The zebra fish (Danio rerio) is a tropical freshwater fish belonging to the minnow family(Cyprinidae) of the order Cypriniformes. Native to the Himalayan region, it is a popular aquarium fish, which is frequently sold under the trade name zebra Danio. Mitochondrial Rho GTPase 2 is an enzyme encoded by the RHOT 2 genes. RHOT2 is a member of the Rho GTPase family and one of two isoforms of the protein Miro: RHOT1 (Miro1) and RHOT2 (Miro2). Probably it is involved in the control of anterograde transport of mitochondria and their sub cellular distribution. Rho GTPase is known to play key roles in the modulation of a wide range of cellular processes like prolifera- tion, apoptosis, cell migration, membrane trafficking, cytoskeleton rearrangements and transcriptional regulation. Through its key role in mitochondrial transport, RHOT2 is involved in mitochondrial homeostasis and apoptosis. On the basis of various structural and physicochemical parameters assessment, 3D structure information of Rho GTPase protein developed. This will help us to know the role of protein in zebra fish and interaction of their ligands. 3D structure prediction of protein require X-ray crystallography and NMR spectroscopy which is very time consuming, tedious method and generate a large amount of data creating a gap between available sequences and solved structure. In silico method for predicting 3D structure reduces this gap. The new predicted structure can also be used for molecular docking studies for more insight into the structure.

5. "Insilico analysis of Recombination activating Gene (RAG1) protein of Labeo Calbasu", Recombination activating gene 1 also known as RAG-1, which is a protein that in humans is encoded by the RAG1 gene. The protein encoded by this gene is involved in activation of immunoglobulin V-D-J recombination. The encoded protein is involved in recognition of the DNA substrate, but stable binding and cleavage activity also requires RAG2. Defects in this gene can be the cause of several diseases. The recombination-activating genes (RAGs) encode enzymes that play an important role in the rearrangement and recombination of the genes of immunoglobulin and T cell receptor molecules. RAG-1 and RAG-2 are essential to the generation of mature B and T lymphocytes, two cell types that are crucial components of the adaptive immune system. RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V (D) J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In silico method for predicting 3D structure can also be used for molecular docking studies for more insight into the structure and the main reason of the study.